产品货号：
GS2087
中文名称：
Bst2UI内切酶
英文名称：
Bst2UI
产品规格：
1kU
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
使用说明
To obtain 100%activity,BSA should be added to the 1x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.?
技术规格
相关搜索：Bst2UI内切酶，Bst2UI限制性内切酶
使用说明
To obtain 100%activity,BSA should be added to the 1x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.?
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1hourat 60℃ in a total reaction volume of 50μl. |
| Recognition sites | CC↑WGG |
| 来源 | Bacillus stearothermophilus 2U |
| Assayed on | Lambda DNA |
| Optimal buffer | G(10mMTris-HCl(pH 7.6 at 25℃);10mMMgCl2;50 mM NaCl;1 mM DTT.)+ BSA |
| Optimal temperature | 60℃ |
| Storage conditions | 10mMTris-HCl(pH 7.5);200 mM NaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;200 μg/ml BSA;50%glycerol.Store at -20℃. |
| Ligations | After Bst2UI overdigestion none of DNA fragmetns can be ligated. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a.of enzyme for 16hoursat 60℃. |
| Methlation effect | Not blocked by overlapping Dcm methylation(CmCWGG):CCWGG. |
| Thermal inactivation | enzyme is inactivated by incubation at 80℃ for 20 mins. |
| Campatible Ends | AjnI?^,BciT130I,BptI,BseBI,BsiLI,BstNI,BstOI,EcoRII^,MvaI,Psp6I?^,PspGI^ |
相关搜索：Bst2UI内切酶，Bst2UI限制性内切酶